Well, we managed to do quite a few things today. We run several PCR reactions with the sample of chromosomal DNA from Drosophila that showed positive on the previous gel. We worked a bit trying to get sequences and design and analyse primers. We poured a gel while we set up some digestions and we finally run everything to see that none of the PCRs worked 
but that the DNAs from my PhD are still “alive”. We also had time to go over the recombinant DNA techniques once more, with an overview of the cloning process that we are pursuing this week, and we also got an introduction to gene expression regulation without getting into the details of the zillion processes that are involved.
but that the DNAs from my PhD are still “alive”. We also had time to go over the recombinant DNA techniques once more, with an overview of the cloning process that we are pursuing this week, and we also got an introduction to gene expression regulation without getting into the details of the zillion processes that are involved.
Running the gel with 9V batteries takes quite a while and I stayed late to let the fragments separate well before cutting. It wasn’t the cleanest of digestions, but at least one of the reactions gave us something to work with. We will purify it tomorrow, for now, it will sleep at 4ºC.
On the left, a faint 1Kb ladder indicates that the fragments of the first lane are ok. I cut the vector (upper band) and the insert (lower band) to continue the cloning exercise tomorrow.
Here you can see the whole left after cutting the lower band.
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